Vol. 1 No.3 September 2015
Facility Cores 
The Comparative Pathology Core  which provides pathology training, laser capture microdissection and pathology assessment of a diverse range of animal models and Systems Technologies Core which provides genomic, metabolic, and proteomic analyses are open for service, consultation and collaboration with CHHE members. Dedicated bioinformatic support is also available. 
Integrative Health Science Facility Core can help you translate your findings from animal/cell models to humans or vice versa.  This core can help you procure human tissues/specimans, help with conduct of human studies (questionnaire development, IRB assistance), use of the comparative toxicogenomic database (CTD, either with specialized training or data curation, assess to clinical populations at ECU, and much more.
Community Outreach and Engagement Core (COEC) to help extend the reach of our research to communities in NC and beyond.
**See the for more information**

This Issue's Core Highlight
CHHE Metabolomics
Dr. Susan Sumner is the leader of the Metabolomics Section of the CHHE Systems Technologies Core.  The CHHE is supported by a collaboration with the NIH Eastern Regional Metabolomics Research Core at RTI International (RTI RCMRC, 1U24DK097193). The RTI RCMRC, directed by Dr. Susan Sumner, is one of six cores, funded by the NIH Common Fund Metabolomics Program, which are working together to establish national standards for metabolomics, to increase national capacity and foster collaborative efforts that will advance translational research, and to facilitate pioneering research and training. Collaborations with the RTI RCMRC include consultation in study deign, sample collection and storage, types of analytical methods to address the study needs, and relevant environmental measures.  The team is experienced in the analysis of endogenous and exogenous compounds in cells, tissues, and biological fluids collected from animal models or human subjects. The RTI RCMRC has the resources and facility to conduct broad spectrum metabolomics analysis using NMR, UPLC-TOF-MS, and 2D-GC-TOF-MS.  The team conducts targeted analysis of metals, as well as metallomics and mineralomics, using ICP-MS, and uses NMR, GC-MS, and LC-MS methods for elucidation of xenobiotic metabolism, and detection of environmentally relevant compounds. 

For more information contact Susan Sumner at RTI

Biosketch Workshop
Biosketch Workshop
On August 17, CHHE hosted a biosketch workshop to help CHHE members and friends create their new NIH biosketch.  Led by librarian, Kris Alpi and her colleagues Bob Sotak and Hilary Davis, we got a hands on opportunity to learn how to link our PubMed bibliography and our federal grant support to create the new required NIH biosketch.  These online tools will also assist in creating the NSF biosketch.    

If you missed it, a video tutorial is at or contact Kris Alpi or the Libraries Ask Us at library_reference@ncsu.eduThe library folks are happy to come to other locations to work with groups or individuals.

CHHE will continue to host training opportunities that benefit all members.  Please send your suggestions to Chris McGahan, Career Development Core Director.

Coming soon: Specific Aims Workshop

Welcome Andy Baltzegar, GSL Director!

Andy Baltzegar is the new Director of the NC State's Genomic Sciences Laboratory (GSL).  Andy is replacing former Director Jenn Schaff who left the GSL to pursue advanced training in bioinformatics. Jenn was also  the Leader of CHHE's Genomic Section of the SystemsTechnologies Facilities Core.  Andy has agreed to be the new Leader of the CHHE Genomic Section and we extend a warm welcome to him and a thanks to Jenn for her commitment to CHHE.  Andy's contact information is 919-513-0738.  Be sure to let the GSL staff know you are a CHHE member so they can track core use.
PINS: Remember when submitting your grants, be sure to select CHHE as a center in PINS.

Research Teams are starting to meet to discuss topics they'd like to work on together and what types of events/enrichment activities they'd like CHHE to offer.

Acknowledgements: Please remember to cite our CHHE P30 grant ES025128 in publications if CHHE has provided you services, facility core use, seed/pilot project funds etc. NIH tracks this and this is an important CHHE metric.
Seminars: Send Jackie seminar announcements/events etc. for the CHHE calendar.

Upcoming CHHE Seminars:
4pm Toxicology Auditorium

Kim Anderson -Developing technologies: Passive wristband samplers assess contaminant mixtures in the personal environment of rural Peruvians, 9/15, see below for more details...

Michael Skinner - Environmentally induced epigenetic transgenerational inheritance of disease and phenotypic variation: Ancestral ghosts in your genome, 10/6

Guillermina Lozano - The p53 tumor suppressor pathway, 11/17
CHHE Website
CHHE website is continuously being updated!  We've added team members to the research team pages - check out your Photo and short research bio!  Please send your bio to Jackie Broughton.  

John Meitzen received an Army Research Office STIR grant for his project: "Validating a mouse tool for analyzing sex differences in striatal neurons and pathologies"

Michael Bereman's novel Multiplex Adduct Peptide Profiling (MAPP)  mass spectrometric method was featured as a paper in the forefront in Analytical Bioanalytical Chemistry   This method allows unbiased detection of protein modifications and was partially funded by a Pilot Project Award Dr. Bereman received in 2014.
Cathrine Hoyo will participate in EPA's Workshop on Epigenetics and Cumulative Risk Assessment on September 1-2, 2015 ( She is also speaking at Leuven University in Belgium on "The development of a cohort for epigenetic studies: case study of the Newborn Epigenetics STudy (NEST)"       

   **Please be sure to send us your "kudos" for our next newsletter**
Send us information on recent grants you have received (title, agency, amount) so we can include it in our next newsletter!

Upcoming Events and Announcements

September 15, 2015 4 pm Toxicology Building Auditorium
Kim A. Anderson, Environmental and Molecular Toxicology, Oregon State University
Developing Exposures, Developing Technologies: Passive Wristband Samplers Assess Contaminant Mixtures in the Personal Environment of Rural Peruvians
Dr. Anderson is a leader in the development of low cost, easy-to-use personal sampling devices for relevant, quantifiable assessment of bioavailable chemical exposure.  She will be giving a seminar on her work in Peru using this technology to measure pesticide and other exposures to rural residents.  Don’t miss this exciting talk!  If you’d like to meet with her while she is here, contact Jane Hoppin.

CHHE 2015 Travel/Workshop Award Announcement--DUE Oct 1, 2015
One of CHHE's mission is support career development of new and early stage investigators. As part of this effort CHHE will provide awards (up to $1,000) to help defray the cost of travel to scientific meetings or small symposia relevant to environmental health science or a workshop that will provide new skills.  Travel/Workshop awards will be reviewed and prioritized by members of the Career Development Core. Your request should be succinct (less than 250 words) and document the importance of attending the requested meeting/workshop. Please also include the entire budget for travel/registration, etc.  Please send your proposals to Jackie Broughton.  We will have another call in the Spring.
October 29, 2015 Ecology & Evolution Burroughs Wellcome Seminar
Gary W. Miller of Emory University’s Rollins School of Public Health.  The title for Dr. Miller’s seminar is The Exposome: Opportunities for Toxicology and will be held from 3:30-4:30 PM in room 101 of David Clark Labs at NC State University.  There will be a reception for students, faculty, and staff to meet Dr. Miller in the Lobby of David Clark Labs preceding the seminar.  There will also be a chance to schedule one-on-one or group discussion time with Dr. Miller on Friday morning, October 30th.

CHHE will host seminar speakers in the fall and spring.  Check them out on the calendar

Congratulations to our 2015 Pilot Project Awardees
Systems toxicology in genetically diverse mouse cells
David L. Aylor (PI), COS
Michael S. Bereman (Co-I), COS

Establishment and implementation of a unique model for elucidating the mechanisms of action of endocrine disrupting compounds during early development
Michael A. Cowley (PI), COS
Mchael S. Bereman (Co-I), COS

Discovery of biomarkers of effect following environmentally-relevant exposure to pharmaceutical pollutants
Jamie C. DeWitt (PI), ECU
Krista McCoy (Co-I), ECU
Eli Hvastkovs (Co-I), ECU

Development and demonstration of methods for measurement of human exposure to air pollution using portable sensors applied to high potential dose scenarios in urban environments
Christopher Frey (PI), COE
Kyle S. Bunds (Co-I), COE
Jonathan M. Casper (Co-I), COE
Andrew P. Grieshop (Co-I), COE

Pesticide exposure assessment of commercial lawn care operators
Travis W. Gannon (PI), CALS
Jane A. Hoppin (Co-I), COS

Immune-suppression by embryonic exposure to polycyclic aromatic hydrocarbons
Jeffrey A. Yoder (PI), CVM
David M. Reif (Co-I), COS

CHHE Publications
Genetic Control of Environmental Variation of Two Quantitative Traits of Drosophila melanogaster Revealed by Whole-Genome Sequencing.
Sørensen P, de Los Campos G, Morgante F, Mackay TF, Sorensen D.
Genetics. 2015 Aug 12. pii: genetics.115.180273. [Epub ahead of print]

Males on demand: the environmental-neuro-endocrine control of male sex determination in daphnids.
LeBlanc GA, Medlock EK.
FEBS J. 2015 Aug 3. doi: 10.1111/febs.13393. [Epub ahead of print]

Mucocutaneous lupus erythematosus in dogs (21 cases).
Olivry T, Rossi MA, Banovic F, Linder KE.
Vet Dermatol. 2015 Aug; 26(4):256-e55. doi: 10.1111/vde.12217. Epub 2015 Jun 12.

Statistical validation of structured population models for Daphnia magna.
Adoteye K, Banks HT, Cross K, Eytcheson S, Flores KB, LeBlanc GA, Nguyen T, Ross C, Smith E, Stemkovski M, Stokely S.
Math Biosci. 2015 Aug; 266:73-84. doi: 10.1016/j.mbs.2015.06.003. Epub 2015 Jun 17.

The Need for Speed: Neuroendocrine Regulation of Socially-controlled Sex Change.
Lamm MS, Liu H, Gemmell NJ, Godwin JR.
Integr Comp Biol. 2015 Aug; 55(2):307-22. doi: 10.1093/icb/icv041. Epub 2015 May 16. Review.

Hormonal programming of rat social play behavior: Standardized techniques will aid synthesis and translation to human health.
Blake BE, McCoy KA.
Neurosci Biobehav Rev. 2015 Aug; 55:184-97. doi: 10.1016/j.neubiorev.2015.04.021. Epub 2015 May 8. Review.

Correlation between maternal and infant cortisol varies by breastfeeding status.
By: Benjamin Neelon, Sara E; Stroo, Marissa; Mayhew, Meghan; Hoyo, Cathrine; et al.
Infant behavior & development Volume: 40   Pages: 252-8   Published: 2015-Aug (Epub 2015 Jul 18)

Multiple window discrete scan statistic for higher-order Markovian sequences
By: Coleman, Deidra A.; Martin, Donald E. K.; Reich, Brian J.
JOURNAL OF APPLIED STATISTICS Volume: 42   Issue: 8   Pages: 1690-1705   Published: AUG 3 2015

Temperature alone does not explain phenological variation of diverse temperate plants under experimental warming
By: Marchin, Renee M.; Salk, Carl F.; Hoffmann, William A.; Dunn, Robert R.; et al.
GLOBAL CHANGE BIOLOGY Volume: 21   Issue: 8   Pages: 3138-3151   Published: AUG 2015

Metabolomic Evaluation of Neutrophilic Airway Inflammation in Cystic Fibrosis
By: Esther, Charles R., Jr.; Coakley, Raymond D.; Henderson, Ashley G.;  Wright, Fred A.; et al.
CHEST Volume: 148   Issue: 2   Pages: 507-515   Published: AUG 2015

Modeling Climate Change Effects on the Height Growth of Loblolly Pine
By: Farjat, Alfredo E.; Isik, Fikret; Reich, Brian J.; et al.
FOREST SCIENCE Volume: 61   Issue: 4   Pages: 703-715   Published: AUG 2015

Machine learning reveals sex-specific 17-estradiol-responsive expression patterns in white perch (Morone americana) plasma proteins
By: Schilling, Justin; Nepomuceno, Angelito I.; Planchart, Antonio; Yoder, Jeffrey A.; et al.
PROTEOMICS Volume: 15   Issue: 15   Pages: 2678-2690   Published: AUG 2015

A marginal cure rate proportional hazards model for spatial survival data
By: Schnell, Patrick; Bandyopadhyay, Dipankar; Reich, Brian J.; et al.

Comparison of toxicity values across zebrafish early life stages and mammalian studies: Implications for chemical testing
By: Ducharme, Nicole A.; Reif, David M.; Gustafsson, Jan-Ake; et al.
REPRODUCTIVE TOXICOLOGY Volume: 55   Special Issue: SI   Pages: 3-10   Published: AUG 1 2015

Mapping burn severity in a disease-impacted forest landscape using Landsat and MASTER imagery
By: Chen, Gang; Metz, Margaret R.; Rizzo, David M.; Meentemeyer, Ross K.; et al.